Quiz on Agricultural Biotechnology

Biotechnology has revolutionized agriculture in many aspects. Think of 'flavr savr' tomato, a transgenic tomato with increased shelf life developed using biotechnology techniques. We developed "golden rice" , rice rich in vitamin A which is designed to feed population affecting with vitamin A deficiency diseases like night blindness. We have unlimited possibilities to make a plant or plant product more desirable using biotechnology techniques. Cell culture and protoplast fusion techniques helped us to develop plants with desirable traits. Intergeneric crosses and production of cybrids have become possible. Biotechnology has helped the production of encapsulated seeds, somaclonal variants, stress and disease resistant plants etc. Techniques of micropropagation have been developed in the case of many crop plants.

1. Which of the following statements about basal promoters is true?
basal promoters can be located in the 3' UTR
basal promoters are necessary for enhancer trapping
basal promters are necessary for heterologous expression
basal promoters are sufficient for expression in the shoot apical meristem
2. Which of the following is involved in seedless watermelon production?
Apomixes
Interspecific crosses
tetraploidy
Apomixes and Interspecific crosses
3. Which of the following is present on a Ti plasmid, but not on any component of a binary vector system?
Vir genes
GUS genes
LB, RB
Opine genes
4. StarLink is most closely associated with which of the following terms?
Cry9
Cry1
Glyphosate
Event 176
5. The quickest way to produce homozyous breeding lines from heterozygous parents is through:
Aneuploidy
half seed technique
doubled haploids
introgression
6. RAPD molecular markers are
recessive
co dominant
dominant
neutral
7. Most commonly used method for transformation of plants is
Protoplast method
Agrobacterium mediated transformation
Microinjection
none of these
8. Star activity of restriction enzyme means
it does not cut at any site
it cuts at only one site
it cuts the restriction site more efficiently
due to change in ionic conc or other componenets it cuts at more sites generating more number of fragments
9. In tissue culture disease resistance can be obtained by
soma clonal variation
Meristem culture
Anther culture
Somatic hybridisation
10. What does the CT value (cutoff threshold) in real time PCR experiments mean?
The lower the value, the higher the transcript amount.
The higher the value, the higher the transcript amount
No transcript, if the value is lower than 30
No transcript, if the value is larger than 30
Score =
Correct answers: