Practice Test on Applied Biology | CSIR UGC NET JRF Exam

1.Which of the following microbial fermentations are anaerobic?
Ethanol and acetone -butanol
Pencillin and Vitamin B12
Streptomycin and Rifampicin
Citric acid and Propionic acid/

2.In tissue culture experiment to initiated shoots from undifferentiated mass of cell the medium must contain
Low auxin and high cytokinin
High auxin and high cytokinin
high auxin and low cytokinin
low auxin and low cytokinin

3.Which microbe has ben used against insect plant pathogens?
Agrobacterium tumefaciens
Agrobacterium rhizogenes
Bacillus thuringenesis
Fusarium nudum

4.To increase the shelf life of tomato, which of the following genes should be silenced?
ACC synthase
EPSP synthase
PEP carboxylase
ABC transporter

5.Which of the following GM crops is the most widely cultivated globally?
Herbicide resistant soyabean
Insect resistant cotton
Insect resistant brinjal
delayed ripening tomato

6.Neomycin phospho-transferase gene, frequently used as selection marker during plant transformation, inactivates which one of the antibiotics?
Hygromycin
Ampicillin
Streptomyci
Kanamycin

7. TILLING is areverse genetics approarch used in functional genomics. Which one of the following is used for TILLING?
T-DNA tagging by Agrobacterium mediated transformation
Transposon tagging using Ac/Ds elements
Mutagenesis with ethylmethane sulphonate
Protoplast transformation by eletroporation

8.Integrity of introduced transgene in mouse can be validated by
Male pronuclear insertion
Fusion of enucleated egg with somatic cells
Transfer into competent embryonic cell
Southern blot analysis

9.To prepare individual tissue cells from a primary culture, the cell- cell and cell matrix interaction must be broken. To achieve this would Not Use:
EDTA
Trypsin
Collagenase
Separase

10.Which is the Best Method for screening of plants for bioprospecting?
Ecological Studies
Ethano driven
Comparison of DNA sequence
Comparison of Protein sequence

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